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COM700: BODY FLUID IDENTIFICATION AND MICROSCOPICAL METHODS OF SPERM DETECTION FOR FORENSIC DNA/SEROLOGY/BIOLOGY
The College of Microscopy is offering this exciting new course on the application of traditional and new methods and techniques of identifying body fluids and sperm in criminal sexual assault evidence. This course introduces DNA analysts, serologists, and biologists to best practices for the identification of human body fluids, blood, saliva, semen, and sperm. Students learn about the different markers, tests, and methods used to identify human body fluids from forensic evidence. Comparative analyses of presumptive vs. confirmatory methods are also included in addition to comprehensive "hands-on" training using commercially available methods. A significant portion of the class focuses on the microscopical identification of sperm using both traditional staining techniques, newer fluorescent methods, and optical contrast techniques such as phase contrast. Hoffman Modulation and Nomarski techniques are also discussed. Both practical and theoretical training on brightfield, fluorescence, and interference microscopy are provided. In addition, the course covers individual sperm isolation techniques via fluorescence stereomicroscopy and laser capture microdissection (LCM).
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No sessions currently scheduled for this course.
Are you interested in this course, but it is either not offered or not at a time that works with your schedule? Let us know and we will notify you when it is available next.
Offline registration is available by calling the registrar at 630-887-7100 or by downloading the offline registration form, completing it, and faxing to 630-887-7412.
* If a scheduled course is full, you may be placed in a waiting list.
- Body Fluid Identification: Identification of saliva, semen, blood, urine, vaginal secretions, menstrual blood, breast milk, cerebrospinal, lachrymal, mucous secretions, and feces.
- Microscopical Methods for Sperm Detection: Brightfield through fluorescence and optical interference.
- Separation Techniques using low tech/low cost fluorescence stereomicroscopy vs. high tech LCM.
- Review of current practices and introduction to new methods of body fluid identification
- Microscopical methods for body fluid identification and detection of spermatozoa.
- Biochemical methods for body fluid identification and confirmation of spermatozoa.
- Methods for the isolation of spermatozoa from smears in order to perform DNA analysis.
- Forensic scientists
- Criminalists
- DNA Analysts
- Serologists
- Detailed course manual including detailed test protocols.
- Each student uses an Olympus BX51 microscope with fluorescence capabilities and a SZX10 stereomicroscope.
- Hands-on experience with all commercially available body fluid tests.
"It presented information that was useful and vital for my job needs". Royal Bahamas Police Force
"Hands-on aspect, I learned techniques I had never seen before". Alaska State Crime Lab
"It was well organized and geared to Forensics. The hands-on experience was helpful and the instructors were very knowledgeable". City of Phoenix Crime Lab
The student is notified at the end of the course whether or not they have successfully completed the requirements of the course based on:
- 100 % attendance
- class participation
- completion of all course material
- completed and signed student evaluation form
- completed pre and post course assessment forms.
Upon successfully meeting these requirements, a student is awarded a certificate of completion and IACET CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or IACET CEU credits.
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