Hooke College


This Infrared Microscopy course is designed to provide practical instruction in real world use of the Fourier transform infrared (FTIR) microscope. The class utilizes demonstrations and laboratory exercises supplemented with lectures. Students are strongly encouraged to bring their own samples for analysis. Class size is limited to twelve students to allow for maximum student participation. Four experienced instructors lead this class, and tailor the laboratory exercises and discussions to the skill level and interests of the students.

This course assumes basic knowledge of infrared spectroscopy and operation of an FTIR microscope. There is a brief discussion of infrared theory, and a heavy emphasis on using FTIR microscopy in problem-solving applications. The emphasis is on student sample preparation exercises and the use of different spectral acquisition methods to obtain optimum results.

Blended Learning This course includes pre- and post-course modules as part of our blended learning program. Click the icon for more information.

Date Times & Locations Instructor(s) Credits Pricing
11/3/2015 -
Westmont, IL
Tues: 8:00 AM-5:00 PM
Wed: 8:00 AM-5:00 PM
Thurs: 8:00 AM-5:00 PM
Martin, Ph.D.
Shearer, Ph.D.
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Course Outline
  1. Introduction and overview of FTIR course and instrumentation
  2. Discussion of student background and interests
  3. Brief review of basic FTIR theory and its relevance to spectral interpretation
  4. FTIR microscope elements and their effect on spectra
  5. What makes a good spectrum and how to obtain one
  6. Sample-specific factors affecting spectral quality, mixtures, detection limits
  7. Discussion and demonstrations of various sample preparation techniques including pyrolysis methods, microextraction techniques, compression methods, and cross-sectioning of laminates
  8. Lab exercises: student preparation of samples and acquisition of spectra
  9. Spectral interpretation
  10. Attenuated Total Reflection (ATR) and direct reflectance methods
  11. Library searches
  12. Additional lectures/lab depending on student interests: Raman spectroscopy, filtration of pharmaceuticals and isolation of filtered particles
  13. Final Review
What You Will Learn
  • Techniques for preparation of various sample types
  • Methods for obtaining the best spectrum possible from real-world samples
  • Perform evaluation of the quality of spectra
Who Should Enroll

This is a practical, hands-on course for new or experienced users who want to improve the quality of their spectral data, and expand the types of samples that they can successfully analyze.

Student Learning Resources
  • Detailed course manual and hand outs
  • Two Thermo-Nicolet Continuum Microscopes attached to Nexus 470 FTIR benches
  • Each student will be assigned to an individual workstation with Olympus SZX-10 stereomicroscope.
  • All instrumentation, materials, and supplies necessary for successful completion of this course will be provided onsite by the Hooke College of Applied Sciences. If you wish to participate in the pre and post- course online web activities associated with this course you must have access to a computer and the internet.
Suggested Prerequisites

Some familiarity with IR spectroscopy.

Satisfactory Completion Requirements

Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. Students are notified at the end of the course whether or not they have successfully completed the requirements of the course based on:

  • 100 % attendance
  • class participation
  • completion of all course material
  • completed and signed student evaluation form
  • completed pre and post course assessment forms.

Upon successfully meeting these requirements, a student is awarded a certificate of completion and CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or CEU credits.

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