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SMP525: SAMPLE PREPARATION: SPERM ISOLATION (FORMERLY COM314)
This one-day practical hands-on course is taught by members of cleanroom staff at McCrone Associates and specializes in the techniques that are used for isolating and mounting spermatozoa from smear slides. The techniques learned in this course are faster and more economical than the equipment required to carry out laser capture micro-dissection. Students first learn how to make the tools that they then apply to the isolation of single spermatozoa. Students apply the technique to samples using an Olympus SZX10 stereomicroscope and an Olympus BX51 polarizing light microscope. More than 50% of the class time is spent practicing the isolation techniques.
A maximum class size of 10 students allows the instructor(s) to provide individual attention. Prior to the course, each enrolled student is asked to fill out a questionnaire and to complete pre-course materials online to ensure their individual needs are met.
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Date
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Times & Locations
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Instructor(s)
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Credits
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Cost
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4/8/2011 -
4/8/2011
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Westmont, IL
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Primozic
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0.5
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Are you interested in this course, but it is either not offered or not at a time that works with your schedule? Let us know and we will notify you when it is available next.
Offline registration is available by calling the registrar at 630-887-7100 or by downloading the offline registration form, completing it, and faxing to 630-887-7412.
* If a scheduled course is full, you may be placed in a waiting list.
- Introduction to stereomicroscopy
- How to make tungsten needles for micro sample preparation
- Microscopy bench supplies
- Creating water soluble gum mounts
- Particle manipulation: techniques, hands
- Particle manipulation: techniques, media
- Introduction to Polarized Light Microscopy
- Dispersion and isolation of sperm heads from sample
- Substrate compatibility for further analysis
- Manual techniques for isolating spermatozoa from rape victim smear slides
- Proper handling of microtools
- Basic Stereomicroscopy using Olympus SZX10
- Basic Polarized Light Microscopy using Olympus BX51
- How to make tungsten needles
- Forensic Scientists
- Criminalists
- DNA Analysts
- Serologists
- Detailed course manual
- Olympus BX51 microscope with fluorescence capabilities and an Olympus SZX10 stereomicroscope.
- Hands-on experience with microtools and isolation techniques.
- Students should be familiar with light microscopy and stereomicroscopy.
Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. In addition, the students are required to have 100% attendance during the course, participate in class, complete a student evaluation form and pre and post course assessment forms.
The student is notified at the end of the course whether or not they have successfully completed the requirements of the course based on:
- 100% attendance
- class participation
- completion of all course material
- completed and signed student evaluation form
- completed pre and post course assessment forms
Upon successfully meeting these requirements, a student is awarded a certificate of completion and IACET CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or IACET CEU credits.
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